Hairy-cell leukaemia: an immunoperoxidase study of paraffin-embedded tissues.

Hairy-cell leukaemia: an immunoperoxidase study of paraffin-embedded tissues.

Paraffin sections of a variety of tissues from 12 patients with typical hairy-cell leukaemia (HCL) were stained for immunoglobulin heavy and light chains by the peroxidase-antiperoxidase (PAP) technique. Plasma cells were frequent, particularly in a lymph node from a severely infected patient. The reactive nature of the plasma cells of HCL was suggested by the fact that there was no restriction...

Diagnosis of hairy-cell leukaemia by tartrate-resistant acid phosphatase activity in paraffin-embedded tissue sections.

Tartrate-resistant acid phosphatase activity may be demonstrated in paraffin-embedded liver and spleen specimens as well as in decalcified bone marrow biopsies after fixation in a mixed glutaraldehyde-formaldehyde-calcium acetate solution. This technique may routinely be applied to haematologically relevant material and aids in the differential diagnosis of hairy-cell leukaemia.

Localisation of enteropathogens in paraffin embedded tissue by immunoperoxidase.

An indirect immunoperoxidase technique has been used to identify enteropathogens in formol-sublimate fixed paraffin embedded sections of calf intestine. Infections with bovine rotavirus, bovine coronavirus, Newbury agent SRV -1, and K99+ Escherichia coli have been detected in the intestines from experimentally infected and conventially reared diarrhoeic or normal calves. The ability to visualiz...

Hairy cell leukaemia.

Hairy cell leukaemia is a rare form of lymphoproliferative disease that affects 2% of leukaemia patients and was first described in 1958 as leukaemic reticuloendotheliosis. The typical presentation is pancytopenia, splenomegaly and abnormal lymphoid cells in blood with hairy cytoplasmic projections. The pathological hairy cell resembles in many ways B-lymphocyte as in chronic lymphocytic leukae...

Laser capture microdissection of paraffin-embedded tissues.